教員情報

イナガキ　ヨシシゲ   INAGAKI YOSHISHIGE   稲垣　善茂    所属   文学部 教育学科    職種   教授
言語種別	英語
発行・発表の年月	2011
形態種別	論文
査読	査読あり
標題	Investigation of the potential for triterpene synthesis in rice through genome mining and metabolic engineering.
執筆形態	共著
掲載誌名	New Phytologist
掲載区分	国外
巻・号・頁	191((2)),pp.432-448
著者・共著者	Inagaki Y, Etherington G, Geisler K, Field B, Ikeda K, Mutsukado Y, Dicks J, Osbourn A
概要	Here, through genome mining and metabolic engineering, we investigate the potential for triterpene synthesis in rice. The first two steps in the oat triterpene pathway are catalysed by a divergent OSC (AsbAS1) and a cytochrome P450 (CYP51). The genes for these enzymes form part of a metabolic gene cluster. To investigate the origins of triterpene synthesis in monocots, we analysed systematically the OSC and CYP51 gene families in rice. We also engineered rice for elevated triterpene content. We discovered a total of 12 OSC and 12 CYP51 genes in rice and uncovered key events in the evolution of triterpene synthesis. We further showed that the expression of AsbAS1 in rice leads to the accumulation of the simple triterpene, beta-amyrin. These findings provide new insights into the evolution of triterpene synthesis in monocots and open up opportunities for metabolic engineering for disease resistance in rice and other cereals.