教員情報

イナガキ　ヨシシゲ   INAGAKI YOSHISHIGE   稲垣　善茂    所属   文学部 教育学科    職種   教授
言語種別	英語
発行・発表の年月	2007
形態種別	論文
査読	査読あり
標題	Elicitin-responsive lectin-like receptor kinase in BY-2 cells.
執筆形態	共著
掲載誌名	DNA Sequence.
掲載区分	国外
巻・号・頁	18,pp.152-159
著者・共著者	Sasabe M, Naitoh K, Suenaga H, Ikeda T, Toyoda K, Inagaki Y, Shiraishi T, Ichinose Y.
概要	The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 ( NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow ( BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein ( GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells.