教員情報

イナガキ　ヨシシゲ   INAGAKI YOSHISHIGE   稲垣　善茂    所属   文学部 教育学科    職種   教授
言語種別	英語
発行・発表の年月	2015
形態種別	論文
査読	査読あり
標題	Characterization of quorum sensing-controlled transcriptional regulator MarR and Rieske (2Fe-2S) cluster-containing protein (Orf5) that are involved in resistance to environmental stresses in Pseudomonas syringae pv. tabaci 6605.
執筆形態	共著
掲載誌名	Mol. Plant Pathol.
掲載区分	国外
巻・号・頁	16(4),pp.376-387
著者・共著者	Taguchi F, Inoue Y, Suzuki T, Inagaki Y, Yamamoto M, Yoyoda K, Noutoshi Y, Shiraishi T, Ichinose Y
概要	To investigate the role of these genes in the virulence of Pta6605, delta marR and delta orf5 mutants were generated. Both mutants showed decreased swimming and swarming motilities, decreased survival ability under oxidative and nitrosative stresses and, consequently, reduced virulence on host tobacco plants. Transmission electron micrographs showed that the structure of the cell membranes of delta marR and delta orf5 mutants was severely damaged. Furthermore, not only the ratio of dead cells, but also the amount of flagella, extracellular DNA and protein released into the culture supernatant, was significantly increased in both mutants, indicating that the disruption of marR and orf5 genes might induce structural changes in the membrane and cell lysis. Because both mutants showed partly similar expression profiles, both gene products might be involved in the same regulatory cascades that are required for QS-dependent survival under environmentally stressed conditions.