教員情報

イナガキ　ヨシシゲ   INAGAKI YOSHISHIGE   稲垣　善茂    所属   文学部 教育学科    職種   教授
言語種別	英語
発行・発表の年月	2014
形態種別	論文
査読	査読あり
標題	Ralstonia solanacearum type III secretion system effector Rip36 induces hypersensitive response in the nonhost wild eggplant Solanum torvum.
執筆形態	共著
掲載誌名	Mol. Plant Pathol.
掲載区分	国外
巻・号・頁	15((3)),pp.297-303
著者・共著者	Nahar K., Matsumoto, I., Taguchi, F., Inagaki, Y., Yamamoto, M., Yoyoda, K., Shiraishi, T., Ichinose, Y., Mukaihara, T.
概要	Recently, 72 effector genes, called rip (Ralstonia protein injected into plant cells), have been identified in R solanacearum RS1000. RS1002, a spontaneous nalixidic acid resistant derivative of RS1000, induced strong HR in the nonhost wild eggplant Solanum torvum in an Hrp dependent manner. An Agrobacterium mediated transient expression system revealed that Rip36, a putative Zn dependent protease effector of R solanacearum, induced HR in S torvum. A mutant in the putative Zn binding motif (E149A) completely abolished the ability to induce HR. In agreement with this result, the RS1002 derived delta rip36 and rip36E149A mutants lost the ability to induce HR in S torvum. An E149A mutant had no effect on the translocation of Rip36 into plant cells. These results induce that Rip36 is an avirulent factor that induces HR in S torvum and that a putative Zn dependent protease motif in essential for this activity.