教員情報

イナガキ　ヨシシゲ   INAGAKI YOSHISHIGE   稲垣　善茂    所属   文学部 教育学科    職種   教授
言語種別	英語
発行・発表の年月	2012
形態種別	論文
査読	査読あり
標題	H2O2 production by copper amine oxidase, a component of the ecto-apyrase (ATPase)-containing protein complex(es) in the pea cell wall, is regulated by an elicitor and a suppressor from Mycosphaerella pinodes.
執筆形態	共著
掲載誌名	J Gen Plant Pathol,
掲載区分	国外
巻・号・頁	78((5)),pp.311-315
著者・共著者	Toyoda K, Yasunaga E, Niwa M, Ohwatari Y, Nakashima A, Inagaki Y, Ichinose Y, Shiraishi T.
概要	Blue native PAGE analysis for cell wall proteins from pea epicotyls demonstrated that cell wall-associated ecto-apyrase (ATPase) formed a large protein complex(es) ranging from 450 to 900 kDa; one of the components of the complex was copper amine oxidase (CuAO), which catalyzes the oxidation of amines with the subsequent generation of ammonia and hydrogen peroxide. CuAO activity was coordinately regulated in vitro with ATP-hydrolyzing activity by an elicitor and a suppressor from Mycosphaerella pinodes. Moreover, treatment of cell wall proteins with the suppressor caused the appearance of the apyrase monomer. On the basis of these results, M. pinodes may target the apyrase-containing protein complex(es) of the host to attenuate cell wall-based, extracellular defense(s) including the production of hydrogen peroxide.