教員情報

イナガキ　ヨシシゲ   INAGAKI YOSHISHIGE   稲垣　善茂    所属   文学部 教育学科    職種   教授
言語種別	英語
発行・発表の年月	2007
形態種別	論文
査読	査読あり
標題	Suppression of Cdc27B induces plant defense responses.
執筆形態	共著
掲載誌名	Mol Plant Pathol.
掲載区分	国外
巻・号・頁	8((4)),pp.365-373
著者・共著者	Kudo C, Suzuki T, Fukuoka S, Asai S, Suenaga H, Sasabe M, Takano Y, Okuno T, Toyoda K, Shiraishi T, Ichinose Y, Inagaki Y*.
概要	Differential-display PCR has revealed that Cdc27B is down-regulated in tobacco BY-2 cells after treatment with INF1 elicitin. Cdc27B is one of 13 essential components of the anaphase-promoting complex or cyclosome (APC/C)-type E3 ubiquitin ligase complex in yeast. This APC/C-type E3 ubiquitin ligase complex regulates G2-to-M phase transition of the cell cycle by proteolytic degradation. In this study, we investigated the roles of this gene, NbCdc27B, in plant defence responses using virus-induced gene silencing. Suppression of NbCdc27B in Nicotiana benthamiana plants induced defence responses and a gain of resistance to Colletotrichum lagenarium fungus. Elicitin-induced hypersensitive cell death (HCD) was inhibited mildly in plants silenced with tobacco rattle virus::Cdc27B. Cdc27B could manage the signalling pathways of plant defence responses as a negative regulator without HCD.